After incubation, the culture medium was collected, centrifuged at 2,000 g for 10 min to remove cellular debris, and the supernatant was carefully harvested for subsequent cytokine quantification using enzyme-linked immunosorbent assays (ELISA) kits: Mouse TNF alpha SimpleStep ELISA Kit (ab208348
Regarding the cellular localization of GLP-1R in the CNS, there are controversial findings
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In nonagenarians, there is a higher expression of macrophage-1 antigen (MAC-1), which is involved in neutrophil adhesion and migration, compared to younger and middle-aged individuals