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During early reperfusion, metformin activates AMPK and promotes endothelial nitric oxide synthase (eNOS) phosphorylation at Ser1177(48), effects abolished in AMPK- or eNOS-deficient mice (Gundewar et al., 2009), confirming pathway dependence

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It was found that the expression of PLC-4 gene in this pathway decreased in cancer, but there was no significant difference in Wnt5a mRNA level