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To explore the potential molecular mechanisms underlying the regulation of GSH metabolism by mitophagy, Signaling Pathways Project predicted TFs associated with 4 up-regulated genes involved in GSH synthesis (Gclm, Gss, Gclc, and Slc7a11) in P3O compared to controls

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After purification of PCR products via magnetic beads, a 200-nucleotide fragment library was prepared on Sentosa SX101

During in vitro testing, the progressive sperm motility in the LC group, CoQ10 group, LC combined with CoQ10 group, and the semen blank control group was 24.3 4.6% and 38.1 5.1%, 23.0 4.8% and 36.9 4.4%, 28.4 5.0% and 43.8 5.4%, 19.7 4.4% and 26.0 4.9%, respectively