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Ideally, these products should be isotonic, but hypertonic solutions (up to 600 mOsm/kg) are sometimes used to reduce injection volume, although higher osmolality can increase pain

Furthermore, neuroprotective and disease-modifying agents, including histone deacetylase 6 (HDAC6) inhibitors and metabolic regulators like metformin, show mechanistic promise but face challenges such as poor solubility, rapid clearance, non-specific distribution, and low brain bioavailability 17,18,19,20

Nutrition needs of mammalian cells in tissue culture

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Noriega-Guerra H, Freitas VM