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Interestingly, the levels of dUMP accumulation reduced over the 72 h despite continued TS inhibition, suggesting that there is rebalancing of the nucleotide pools to resolve stress

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Because of this, if applied to intact cells or tissues, this method requires cell lysis which is usually carried out by acidification with consequent onset of large amounts of GSSG as an artifact

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That gap shows why food alone rarely moves your levels much