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Both were incubated overnight at 37 C, without CO 2

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2.5 mg = 0.5 mL = 50 units 5 mg = 1.0 mL = 100 units (a full 1 mL U-100 syringe) 7.5 mg = 1.5 mL = 150 units (needs two draws on a 1 mL syringe) 10 mg = 2.0 mL = 200 units 12.5 mg = 2.5 mL = 250 units When to re-check the math Recalculate when you change vial size, BAC water volume, dose, syringe type, or syringe capacity