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Some promising compounds based on in vitro and in vivo testing fail during human clinical trials due to the species-specific differences in pharmacokinetics and toxicology [267]

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The plate was incubated in the dark for 60 min at room temperature (22 C) and subsequently read on an EnVision Multilabel Reader (PerkinElmer Life and Analytical Sciences), with excitation at 340 nm and measurements of emission at 615 and 665 nm