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After 48 h, cleaved two-cell embryos from each group were microscopically observed with cleavage rates calculated as the percentage of cleaved two-cell embryos/MII oocytes

Next, left hemispheres were washed for 10 min three times in PBS at room temperature, followed by cryopreservation via placement in a 15 mL conical tube containing 30% sucrose in PBS until the tissues sunk to the bottom

Thus, it is speculated that Ipamorelin might influence food consumption by interacting with ghrelin receptors in the nervous system

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