Superoxide measurements in liver and kidney by electron paramagnetic resonance (EPR) spectroscopy Mitochondrial superoxide in liver and kidney tissue was measured by EPR spectroscopy using a mitochondrial targeted probe, 1-hydroxy-4-[2-triphenylphosphonio)-acetamido]-2,2,6,6-tetramethylpiperidine,1-Hydroxy-2,2,6,6-tetramethyl-4-[2-(triphenylphosphonio)acetamido] piperidinium dichloride (mito-TEMPO-H) (Enzo Life Sciences, Farmingdale, NY) 24,25
Metabolic phenotyping For the glucose tolerance tests, mice were fasted for 2 h (beginning at ZT0) and then administered glucose via oral gavage (2.5 g kg 1 body weight)
Subcutaneous injection in the abdomen (at least two inches away from the navel), the front of the thigh, or the back of the upper arm
Cng dng kem dng trng da Glutathione Dng m v gip da lun trong trng thi nc, cng mng
IGF-1 is also produced locally in all bodily tissues [92]