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Approximately 80% of patients with COPD in the United States are current or previous smokers so it is easy to see why people think only smokers get COPD

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Lung tissues were collected and homogenized in a lysis buffer (Cell lysis buffer: 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na 2 EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM, -glycerophosphate, 1 mM Na 3 VO 4 , and 1 g/ml leupeptin

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