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Description

Complete RPMI phenol red-free media (Gibco) was used and was beforehand completed with CellTox Green Dye at a 1:500 ratio (v/v)

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In one study, pressure inside a spinal disc dropped to below 100 mm Hg when VAX-D was applied, showing that the treatment can effectively decompress the discs (Ramos et al., 1994)

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