Cells were seeded on collagen-coated plates (250 g/ml rat collagen, Roche) at a density of 150,000 cells/cm 2 and cultivated for 3 h in Williams E medium (PAN Biotech) containing penicillin (100 U/ml, Gibco), streptomycin (0.1 mg/ml, Gibco), gentamycin (10 M, PAN Biotech), dexamethasone (100 nM, Sigma), stable l-glutamine (2 mM, Sigma), insulin supplement (2 ng/ml, Sigma), and 10% Sera Plus (PAN Biotech)
The solution is ready to drink directly from the bottle
A randomized phase II trial of MEK and CDK4/6 inhibitors vesus tipiracil/trifluridine (TAS-102) in metastatic KRAS/NRAS mutant (mut) colorectal cancer (CRC)
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Glutathione preferentially reacts with Ag + ions (due to its high metal ion affinity) and inhibits the production of OxOPD, resulting in a change in FRET peaks
