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GSH biosynthesis occurs exclusively in the cytosol via a two-step ATP-dependent reaction catalysed sequentially by two enzymes: gamma-glutamylcysteine synthetase (GCL also referred to as gamma-glutamylcysteine ligase, comprising the catalytic GCLC subunit and regulatory GCLM subunit), which catalyses the rate-limiting first step of joining glutamate and cysteine

Every day, your liver filters hundreds of gallons of blood, removing waste and processing nutrients

Notably, both its loss and uncontrolled overexpression are destructive, highlighting the need for precise regulation to maintain cardiac metabolism [142, 143]

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