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Sections were stained with hematoxylin and eosin (H&E), wheat germ agglutinin (FITC-conjugated, Merck Life Science Oy, UK) and Picro-Sirius red (Abcam, # ab245887) to measure cell size and cardiac fibrosis level in the LV free wall at the level of the papillary muscles

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DSIP was found to be present in elevated concentrations during slow-wave, or delta, sleep phases