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Materials and Methods RAW264 Cells, GSH Depletion, and Treatment RAW264 cells were cultured in Roswell Park Memorial Institute medium 1640 with 2 mM l-glutamine (Sigma), 100 U/ml penicillin, 100 g/ml streptomycin sulfate (Invitrogen/Life Technologies), and 10% heat-inactivated FCS (Sigma)-omplete medium

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Another pre-clinical study done on C57BL/6J mice using abdominal irradiation (10 Gy) found that the expression level of miR-34a-5p in the small intestine tissue and peripheral blood significantly increased, which mediated distant cognition impairment