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All qRTPCR assays were performed on the QuantStudio 5 Real-Time PCR system (Applied Biosystems, CA, USA) with Power-Up SYBR Master Mix (Applied Biosystems, CA, USA)

For antigen retrieval, the slides were placed in 10 mM sodium citrate buffer (pH 6.0) and heated to near-boiling (9598C) in a water bath for 20 min

Currently, there is too immature an understanding of PD pathophysiology to make vagotomy a potential treatment method, and clarification through research is needed regarding the vagotomy-PD relationship

The Bottom Line The desire and need for better symptomatic therapies and certainly for a disease-modifying treatment for those living with PD is more than understandable

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