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Histological damage was semi-quantitatively scored (1 = minimal change, 5 = severe change) for muscle fiber degeneration, edema, inflammatory infiltration, and vascular congestion using a light microscope (Leica DM4000B, Germany) according to previously described methods.31,73 All histopathological and immunohistochemical evaluations were performed by a pathologist blinded to the experimental groups

The base-labile Fmoc group was removed from the N-terminus of the growing peptide chain by agitation with 20% v / v piperidine in DMF (15 mL) for 5 min, which was then drained and repeated for a further 15 min

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